Library / Reference Chips / 50 µL / 500 µm reaction chamber — our footprint (Fluidic 842)

50 µL / 500 µm reaction chamber — our footprint (Fluidic 842)

chamberchip_f842

reference chip Phrozen Sonic Mighty 12K design, checked in CAD chamber-chip 50 µL / 500 µm

Ali Tahmasbizadeh, Gale Lab, University of Utah

50 µL / 500 µm reaction chamber — our footprint (Fluidic 842). A computer render of the design, not a photograph: its body drawn see-through, its channels in orange. The chip is 34.2 mm × 9.32 mm × 2.3 mm. Beside it, its cross-section across the middle.
Rendered from its design files, not photographed: the body see-through, the channels orange, and its cross-section beside it. Pictures in one family share one scale.

Internal geometry — X-ray

X-ray see-through view of 50 µL / 500 µm reaction chamber — our footprint (Fluidic 842): plan footprint (true scale) and z-exaggerated isometric of the internal chamber, feed channels and two bores

See-through internal view. Left: fluid footprint in plan, true scale (teal); ports show as the wider circles. Right: isometric of the internal void, vertical z exaggerated 3× for visibility. The exterior is an opaque solid block — this is what is inside it.

Download: PNG (x-ray)

Longitudinal section & exterior

Longitudinal section at y=0 (left): grey = solid, white = fluid voids. Exterior isometric (right): the outside of the CAD model, drawn opaque. Click either for full resolution.

Download: PNG (section) · PNG (top) · PNG (iso)

Description

A single monolithic resin-SLA block with one 50 µL, 500 µm-deep stadium reaction chamber, two straight 0.5 mm feed channels on the same floor, and two Ø1.5 mm vertical access bores to the top surface. The vendor part is a sealed polymer body — this is a different monolithic construction that reproduces the chamber's fluidic spec only. Footprint 34.1686 × 9.3246 × 2.3 mm.

The footprint here is ours, not the vendor’s. microfluidic ChipShop publishes only the chamber volume (50 µL) and its depth (500 µm). Those two numbers are the functional specification of a reaction chamber and the model reproduces them exactly — the mesh measures 49.9888 µL, -0.022% from target. The shape is not on record. This stadium comes from an inverse-solve: area = volume ÷ depth = 100 mm², then a width chosen to hold the same stadium aspect ratio (L/w = 2.7146) as chamberchip_f584 — that is w = √(A/2.5) = 6.3246 mm, giving a chamber length of 17.1686 mm. The width is ours; only the volume and depth are the vendor’s. It will not fit a ChipShop holder and is not dimensionally interchangeable with the vendor part.

Verification

metricvalue
Chamber volume — vendor target50 µL
Chamber volume — measured on the model's mesh49.9888 µL (-0.022% vs target)
Chamber depth — vendor500 µm
Chamber area (volume ÷ depth)100 mm²
Chamber stadium (L × w) — OUR design17.1686 × 6.3246 mm OUR DESIGN
Stadium aspect ratio (L/w)2.7146 — held from chamberchip_f584 so the family is geometrically similar
Footprint (L × W × H) — OUR design34.1686 × 9.3246 × 2.3 mm OUR DESIGN
Chip watertightTrue
Chip Euler number0 — genus-1: one cavity, two vents
Void connected bodies1 — chamber and both bores continuous
Min feature vs Phrozen 12K X (px_min = 500/19)26.32 px
Min feature vs Phrozen 12K Y (px_min = 500/24)20.83 px
Min feature vs BYU Nordin (px_min = 500/7.6)65.79 px

All geometry checks run with trimesh (merged vertices) on the exported STLs. See the bundled README for the full method.

Cross-family consistency. This inverse-solve reproduces the vendor volume to -0.022% here, and to the same −0.022% on every other chamber in this family — f843 (2.5 µL), f750 (5 µL), f585 (10 µL), f559 (20 µL), f842 (50 µL) and the original f584 (20 µL). Agreement to three decimal places across a 20× volume range is the only cross-check available for parts the vendor describes by volume alone, and it shows the solve behaves identically at every scale.

Provenance & caveats

Vendor-attributed: chamber volume (50 µL) and chamber depth (500 µm) — product page. Nothing else. OURS (not the vendor’s): the chamber shape, the whole footprint, the feed-channel geometry and the port style. Ports are simplified straight cylindrical bores, standing in for the vendor’s MFCS Mini Luer sockets. Printability: px_min = 26.32 px in X and 20.83 px in Y on the Phrozen Sonic Mighty 12K (19 × 24 µm), and 65.79 px on the BYU Nordin printer (7.6 µm/px). These are pixel counts; for the process limits on record for each printer, see Hardware and process. Status — Phrozen Sonic Mighty 12K design, checked in CAD: the geometry is watertight and volumetrically checked in CAD — see the Verification table above.

Files to download

  • SCADchamberchip_f842.scad12.5 KBDownload
  • STLchamberchip_f842.stl208.9 KBDownload
  • STLvoid_chamberchip_f842.stl206.0 KBDownloadFor inspection, not for printing: the fluid network, every space that holds liquid, as a solid.
  • PNGchamberchip_f842_xray.png170.3 KBDownload
  • PNGchamberchip_f842_section.png23.3 KBDownload
  • MDREADME.md1.7 KBDownload

Tags

  • chamber-chip
  • not-tested
  • reaction-chamber
  • adaptation
  • chipshop-adapted
  • sla-print
  • continuous-flow
  • platform-agnostic
  • scad-source
  • stl-included

Provenance & license

Ali Tahmasbizadeh, Gale Lab, University of Utah

SLA adaptation. Chamber volume and depth taken from the microfluidic ChipShop product page; the chamber shape and the chip footprint are our design, derived by inverse-solve. Monolithic 3D-print adaptation, NOT a vendor part.

License: MIT