Library / Reference Chips / 50 µL chamber chip, 500 µm deep

50 µL chamber chip, 500 µm deep

chamber_50ul_500um

reference chip Phrozen Sonic Mighty 12K design, checked in CAD chamber-chip 50 µL / 500 µm

Ali Tahmasbizadeh, Gale Lab, University of Utah

50 µL chamber chip, 500 µm deep. A computer render of the design, not a photograph: its body drawn see-through, its channels in orange. The chip is 34.2 mm × 9.32 mm × 2.3 mm. Beside it, its cross-section across the middle.
Rendered from its design files, not photographed: the body see-through, the channels orange, and its cross-section beside it. Pictures in one family share one scale.

Internal geometry — X-ray

X-ray see-through view of 50 µL chamber chip, 500 µm deep: plan footprint (true scale) and z-exaggerated isometric of the internal chamber, feed channels and two bores

See-through internal view. Left: fluid footprint in plan, true scale (teal); ports show as the wider circles. Right: isometric of the internal void, vertical z exaggerated 3× for visibility. The exterior is an opaque solid block — this is what is inside it.

Download: PNG (x-ray)

Longitudinal section & exterior

Longitudinal section at y=0 (left): grey = solid, white = fluid voids. Exterior isometric (right): the outside of the CAD model, drawn opaque. Click either for full resolution.

Download: PNG (section) · PNG (top) · PNG (iso)

Description

A single monolithic resin-SLA block with one 50 µL, 500 µm-deep stadium reaction chamber, two straight 0.5 mm feed channels on the same floor, and two Ø1.5 mm vertical access bores to the top surface. Footprint 34.1686 × 9.3246 × 2.3 mm.

The target is a 50 µL chamber, 500 µm deep. The model reproduces it exactly — the mesh measures 49.9888 µL, -0.022% from target. The stadium is sized by an inverse-solve: area = volume ÷ depth = 100 mm², then a width chosen to hold the same stadium aspect ratio (L/w = 2.7146) as the 20 µL chamber chip, 500 µm deep — that is w = √(A/2.5) = 6.3246 mm, giving a chamber length of 17.1686 mm.

Verification

metricvalue
Chamber volume — target50 µL
Chamber volume — measured on the model's mesh49.9888 µL (-0.022% vs target)
Chamber depth — target500 µm
Chamber area (volume ÷ depth)100 mm²
Chamber stadium (L × w)17.1686 × 6.3246 mm
Stadium aspect ratio (L/w)2.7146 — held from chamber_20ul_500um so the family is geometrically similar
Footprint (L × W × H)34.1686 × 9.3246 × 2.3 mm
Chip watertightTrue
Chip Euler number0 — genus-1: one cavity, two vents
Void connected bodies1 — chamber and both bores continuous
Min feature vs Phrozen 12K X (px_min = 500/19)26.32 px
Min feature vs Phrozen 12K Y (px_min = 500/24)20.83 px
Min feature vs BYU Nordin (px_min = 500/7.6)65.79 px

All geometry checks run with trimesh (merged vertices) on the exported STLs. See the bundled README for the full method. The minimum-feature rows are pixel counts; for each printer’s process limits, see Hardware and process.

Cross-family consistency. This inverse-solve reproduces the target volume to -0.022% here, and to within 0.023% on every chamber in this family — the 2.5, 5, 10 and 50 µL chambers and the 20 µL chambers 350 and 500 µm deep. Agreement to three decimal places across a 20× volume range shows the solve behaves identically at every scale.

Credits

Design: Ali Tahmasbizadeh, Gale Lab, University of Utah.

Files to download

  • SCADchamber_50ul_500um.scad12.1 KBDownload
  • STLchamber_50ul_500um.stl208.9 KBDownload
  • STLvoid_chamber_50ul_500um.stl206.1 KBDownloadFor inspection, not for printing: the fluid network, every space that holds liquid, as a solid.
  • PNGchamber_50ul_500um_xray.png148.2 KBDownload
  • PNGchamber_50ul_500um_section.png19.4 KBDownload
  • MDREADME.md1.4 KBDownload

Tags

  • chamber-chip
  • not-tested
  • reaction-chamber
  • sla-print
  • continuous-flow
  • platform-agnostic
  • scad-source
  • stl-included

Citation

Ali Tahmasbizadeh, Gale Lab, University of Utah

Monolithic SLA design: a 50 µL reaction chamber, 500 µm deep, its stadium sized from the target volume and depth.