# chamber_20ul_350um — reference-chip bundle (chamber-chip family)

One stadium reaction chamber, 20 µL at 350 µm depth, with two
straight 0.5 mm feed channels and two Ø1.5 mm vertical access bores, in a monolithic
resin-SLA block.

## Credits

- Design: Ali Tahmasbizadeh, Gale Lab, University of Utah.
- Target: chamber volume 20 µL, depth 350 µm. The stadium is sized from the
  volume and depth: area = volume/depth = 57.1429 mm², width w = sqrt(A/2.5) = 4.7809 mm
  (holding the aspect ratio of the 20 µL, 500 µm chamber, L/w = 2.7146), length
  12.9783 mm.
- The ports are straight bores.

## Verification (trimesh, merged vertices)

| metric | value |
|---|---|
| chamber volume — target | 20 µL |
| chamber volume — mesh | 19.9955 µL (-0.022%) |
| footprint | 29.9782 × 7.7809 × 2.15 mm |
| chip watertight / Euler | True / 0 |
| void connected bodies | 1 |
| px_min @ 19 µm/px (Phrozen 12K, X) | 18.42 px |
| px_min @ 24 µm/px (Phrozen 12K, Y) | 14.58 px |
| px_min @ 7.6 µm/px (BYU Nordin) | 46.05 px |
| tier | good |

**Cross-family consistency:** the same inverse-solve reproduces the target volume to
within 0.023% on every chamber in this family (2.5 / 5 / 10 / 20 / 50 µL, and the
20 µL chamber 500 µm deep). Agreement across a 20× volume range shows the solve behaves
the same at every scale.

**Phrozen Sonic Mighty 12K design** — geometry verified in CAD.